PROSITE: PDOC00113 (documentation file)

ExPASy Home page | Site Map | Search ExPASy | Contact us | PROSITE

Mirror sites: | Australia | Canada | China | Taiwan

# PROSITE: PDOC00113 (documentation)

    {PDOC00113}
    {PS00123; ALKALINE_PHOSPHATASE}
    {BEGIN}
    ************************************
    * Alkaline phosphatase active site *
    ************************************

    Alkaline phosphatase (EC 3.1.3.1) (ALP) [1] is a zinc and magnesium-containing
    metalloenzyme  which hydrolyzes phosphate esters, optimally at high pH.  It is
    found in nearly  all living organisms,  with the exception of some plants.  In
    Escherichia coli, ALP (gene phoA) is found in the periplasmic space.  In yeast
    it (gene  PHO8)  is  found  in  lysosome-like vacuoles and in mammals, it is a
    glycoprotein attached to the membrane by a GPI-anchor.

    In mammals, four different isozymes are currently known [2]. Three of them are
    tissue-specific:  the  placental,  placental-like (germ cell)   and intestinal
    isozymes.  The fourth form is  tissue non-specific and was previously known as
    the liver/bone/kidney isozyme.

    Streptomyces' species  involved  in  the  synthesis  of  streptomycin (SM), an
    antibiotic, express  a  phosphatase (EC 3.1.3.39) (gene strK) which is  highly
    related to ALP.   It specifically cleaves  both  streptomycin-6-phosphate and,
    more slowly, streptomycin-3"-phosphate.

    A serine is involved   in the catalytic activity of ALP. The region around the
    active site serine is relatively well conserved and can be used as a signature
    pattern.

    -Consensus pattern: [IV]-x-D-S-[GAS]-[GASC]-[GAST]-[GA]-T
                        [S is the active site residue]
    -Sequences known to belong to this class detected by the pattern: ALL.
    -Other sequence(s) detected in SWISS-PROT: 3.
    -Last update: June 1994 / Text revised.

    [ 1] Trowsdale J., Martin D., Bicknell D., Campbell I.
         Biochem. Soc. Trans. 18:178-180(1990).
    [ 2] Manes T., Glade K., Ziomek C.A., Millan J.L.
         Genomics 8:541-554(1990).
    [ 3] Mansouri K., Piepersberg W.
         Mol. Gen. Genet. 228:459-469(1991).

    +----------------------------------------------------------------------------+
    | This PROSITE entry  is copyright  by the Swiss Institute of Bioinformatics |
    | (SIB).  There are no restrictions on its use by non-profit institutions as |
    | long as its  content  is  in  no  way  modified  and this statement is not |
    | removed. Usage by and for commercial entities requires a license agreement |
    | (See http://www.isb-sib.ch/announce/ or email to license@isb-sib.ch).      |
    +----------------------------------------------------------------------------+
    {END}

ExPASy Home page | Site Map | Search ExPASy | Contact us | PROSITE

Mirror sites: | Australia | Canada | China | Taiwan