BLASTX 2.0a19MP-WashU [05-Feb-1998] [Build sol2.5-ultra 01:47:30 05-Feb-1998]

Reference:  Gish, Warren (1994-1997).  unpublished.
Gish, Warren and David J. States (1993).  Identification of protein coding
regions by database similarity search.  Nat. Genet. 3:266-72.

Notice:  statistical significance is estimated under the assumption that the
equivalent of one entire reading frame in the query sequence codes for protein
and that significant alignments will involve only coding reading frames.

Query=  U51677
        (2575 letters)

  Translating both strands of query sequence in all 6 reading frames

Database:  swissprot
           72,615 sequences; 26,335,942 total letters.
Searching....10....20....30....40....50....60....70....80....90....100% done

                                                                     Smallest
                                                                       Sum
                                                     Reading  High  Probability
Sequences producing High-scoring Segment Pairs:        Frame Score  P(N)      N

sp|P09429|HMG1_HUMAN HIGH MOBILITY GROUP PROTEIN HMG1 ... +2   311  5.0e-108  4
sp|P10103|HMG1_BOVIN HIGH MOBILITY GROUP PROTEIN HMG1 ... +2   311  1.3e-107  4
sp|P07155|HMG1_MOUSE HIGH MOBILITY GROUP PROTEIN HMG1 ... +2   311  2.7e-107  4
sp|P12682|HMG1_PIG   HIGH MOBILITY GROUP PROTEIN HMG1 ... +2   306  4.6e-107  4



>sp|P09429|HMG1_HUMAN HIGH MOBILITY GROUP PROTEIN HMG1 (HMG-1).
            Length = 214

  Plus Strand HSPs:

 Score = 311 (109.5 bits), Expect = 5.0e-108, Sum P(4) = 5.0e-108
 Identities = 58/58 (100%), Positives = 58/58 (100%), Frame = +2

Query:   971 SAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEK 1144
             SAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEK
Sbjct:    99 SAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEK 156

 Score = 303 (106.7 bits), Expect = 5.0e-108, Sum P(4) = 5.0e-108
 Identities = 59/59 (100%), Positives = 59/59 (100%), Frame = +1

Query:  2146 KDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEEEDEEDEDEEEDDDDE 2322
             KDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEEEDEEDEDEEEDDDDE
Sbjct:   156 KDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEEEDEEDEDEEEDDDDE 214

 Score = 276 (97.2 bits), Expect = 5.0e-108, Sum P(4) = 5.0e-108
 Identities = 50/54 (92%), Positives = 50/54 (92%), Frame = +1

Query:     4 GKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKVRGLK 165
             GKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWK    K
Sbjct:     1 GKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSAK 54

 Score = 265 (93.3 bits), Expect = 5.0e-108, Sum P(4) = 5.0e-108
 Identities = 52/56 (92%), Positives = 52/56 (92%), Frame = +3

Query:   330 TMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPP*VSCLFL 497
             TMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPP  S  FL
Sbjct:    50 TMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPP--SAFFL 103

 Score = 80 (28.2 bits), Expect = 1.8e-31, Sum P(2) = 1.8e-31
 Identities = 18/60 (30%), Positives = 30/60 (50%), Frame = +2

Query:   971 SAFFLFCSEYRPKIKGEHPGLSIG--DVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEK 1144
             S++  F    R + K +HP  S+   + +KK  E W   +A +K  +E  A   K +YE+
Sbjct:    13 SSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSAKEKGKFEDMAKADKARYER 72

 Score = 65 (22.9 bits), Expect = 6.7e-30, Sum P(2) = 6.7e-30
 Identities = 14/45 (31%), Positives = 22/45 (48%), Frame = +1

Query:    13 DPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERW 147
             DP  P+   S++  F    R + K +HP  S+   + +KK  E W
Sbjct:    90 DPNAPKRPPSAFFLFCSEYRPKIKGEHPGLSIG--DVAKKLGEMW 132

 Score = 42 (14.8 bits), Expect = 1.3e-24, Sum P(2) = 1.3e-24
 Identities = 11/37 (29%), Positives = 19/37 (51%), Frame = +3

Query:   336 SAKEKGKFEDMAKADKARYEREMKTY-IPPKGETKKK 443
             +A +K  +E  A   K +YE+++  Y    K +  KK
Sbjct:   136 AADDKQPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKK 172


>sp|P10103|HMG1_BOVIN HIGH MOBILITY GROUP PROTEIN HMG1 (HMG-1).
            Length = 214

  Plus Strand HSPs:

 Score = 311 (109.5 bits), Expect = 1.3e-107, Sum P(4) = 1.3e-107
 Identities = 58/58 (100%), Positives = 58/58 (100%), Frame = +2

Query:   971 SAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEK 1144
             SAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEK
Sbjct:    99 SAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEK 156

 Score = 299 (105.3 bits), Expect = 1.3e-107, Sum P(4) = 1.3e-107
 Identities = 58/59 (98%), Positives = 59/59 (100%), Frame = +1

Query:  2146 KDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEEEDEEDEDEEEDDDDE 2322
             KDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEEEDEEDE+EEEDDDDE
Sbjct:   156 KDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEEEDEEDEEEEEDDDDE 214

 Score = 276 (97.2 bits), Expect = 1.3e-107, Sum P(4) = 1.3e-107
 Identities = 50/54 (92%), Positives = 50/54 (92%), Frame = +1

Query:     4 GKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKVRGLK 165
             GKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWK    K
Sbjct:     1 GKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSAK 54

 Score = 265 (93.3 bits), Expect = 1.3e-107, Sum P(4) = 1.3e-107
 Identities = 52/56 (92%), Positives = 52/56 (92%), Frame = +3

Query:   330 TMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPP*VSCLFL 497
             TMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPP  S  FL
Sbjct:    50 TMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPP--SAFFL 103

 Score = 80 (28.2 bits), Expect = 4.9e-31, Sum P(2) = 4.9e-31
 Identities = 18/60 (30%), Positives = 30/60 (50%), Frame = +2

Query:   971 SAFFLFCSEYRPKIKGEHPGLSIG--DVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEK 1144
             S++  F    R + K +HP  S+   + +KK  E W   +A +K  +E  A   K +YE+
Sbjct:    13 SSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSAKEKGKFEDMAKADKARYER 72

 Score = 65 (22.9 bits), Expect = 1.8e-29, Sum P(2) = 1.8e-29
 Identities = 14/45 (31%), Positives = 22/45 (48%), Frame = +1

Query:    13 DPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERW 147
             DP  P+   S++  F    R + K +HP  S+   + +KK  E W
Sbjct:    90 DPNAPKRPPSAFFLFCSEYRPKIKGEHPGLSIG--DVAKKLGEMW 132

 Score = 42 (14.8 bits), Expect = 1.3e-24, Sum P(2) = 1.3e-24
 Identities = 11/37 (29%), Positives = 19/37 (51%), Frame = +3

Query:   336 SAKEKGKFEDMAKADKARYEREMKTY-IPPKGETKKK 443
             +A +K  +E  A   K +YE+++  Y    K +  KK
Sbjct:   136 AADDKQPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKK 172


>sp|P07155|HMG1_MOUSE HIGH MOBILITY GROUP PROTEIN HMG1 (HMG-1) (AMPHOTERIN)
            (HEPARIN-BINDING PROTEIN P30).
            Length = 214

  Plus Strand HSPs:

 Score = 311 (109.5 bits), Expect = 2.7e-107, Sum P(4) = 2.7e-107
 Identities = 58/58 (100%), Positives = 58/58 (100%), Frame = +2

Query:   971 SAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEK 1144
             SAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEK
Sbjct:    99 SAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEK 156

 Score = 296 (104.2 bits), Expect = 2.7e-107, Sum P(4) = 2.7e-107
 Identities = 57/59 (96%), Positives = 59/59 (100%), Frame = +1

Query:  2146 KDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEEEDEEDEDEEEDDDDE 2322
             KDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEE+DEEDEEDEEEEE+EEDEDEEEDDDDE
Sbjct:   156 KDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEDDEEDEEDEEEEEEEEDEDEEEDDDDE 214

 Score = 276 (97.2 bits), Expect = 2.7e-107, Sum P(4) = 2.7e-107
 Identities = 50/54 (92%), Positives = 50/54 (92%), Frame = +1

Query:     4 GKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKVRGLK 165
             GKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWK    K
Sbjct:     1 GKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSAK 54

 Score = 265 (93.3 bits), Expect = 2.7e-107, Sum P(4) = 2.7e-107
 Identities = 52/56 (92%), Positives = 52/56 (92%), Frame = +3

Query:   330 TMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPP*VSCLFL 497
             TMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPP  S  FL
Sbjct:    50 TMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPP--SAFFL 103

 Score = 114 (40.1 bits), Expect = 1.4e-57, Sum P(3) = 1.4e-57
 Identities = 25/63 (39%), Positives = 43/63 (68%), Frame = +1

Query:  2146 KDIAAYRAKGKPD-AAKKGVVKAEKSKK---KKEEEEDEEDEEDEEEEEDEEDEDEEEDD 2313
             K  A  + K + D AA +   K + +KK   K E+ + +++EED+EE+E++E+E+EEE+D
Sbjct:   145 KKAAKLKEKYEKDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEDDEEDEEDEEEEEEEED 204

Query:  2314 DDE 2322
             +DE
Sbjct:   205 EDE 207

 Score = 80 (28.2 bits), Expect = 1.0e-30, Sum P(2) = 1.0e-30
 Identities = 18/60 (30%), Positives = 30/60 (50%), Frame = +2

Query:   971 SAFFLFCSEYRPKIKGEHPGLSIG--DVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEK 1144
             S++  F    R + K +HP  S+   + +KK  E W   +A +K  +E  A   K +YE+
Sbjct:    13 SSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSAKEKGKFEDMAKADKARYER 72

 Score = 65 (22.9 bits), Expect = 3.8e-29, Sum P(2) = 3.8e-29
 Identities = 14/45 (31%), Positives = 22/45 (48%), Frame = +1

Query:    13 DPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERW 147
             DP  P+   S++  F    R + K +HP  S+   + +KK  E W
Sbjct:    90 DPNAPKRPPSAFFLFCSEYRPKIKGEHPGLSIG--DVAKKLGEMW 132

 Score = 42 (14.8 bits), Expect = 1.3e-24, Sum P(2) = 1.3e-24
 Identities = 11/37 (29%), Positives = 19/37 (51%), Frame = +3

Query:   336 SAKEKGKFEDMAKADKARYEREMKTY-IPPKGETKKK 443
             +A +K  +E  A   K +YE+++  Y    K +  KK
Sbjct:   136 AADDKQPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKK 172


>sp|P12682|HMG1_PIG HIGH MOBILITY GROUP PROTEIN HMG1 (HMG-1).
            Length = 214

  Plus Strand HSPs:

 Score = 306 (107.7 bits), Expect = 4.6e-107, Sum P(4) = 4.6e-107
 Identities = 57/58 (98%), Positives = 57/58 (98%), Frame = +2

Query:   971 SAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEK 1144
             SAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDK PYEKKAAKLKEKYEK
Sbjct:    99 SAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKHPYEKKAAKLKEKYEK 156

 Score = 299 (105.3 bits), Expect = 4.6e-107, Sum P(4) = 4.6e-107
 Identities = 58/59 (98%), Positives = 59/59 (100%), Frame = +1

Query:  2146 KDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEEEDEEDEDEEEDDDDE 2322
             KDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEEEDEEDE+EEEDDDDE
Sbjct:   156 KDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEEEDEEDEEEEEDDDDE 214

 Score = 276 (97.2 bits), Expect = 4.6e-107, Sum P(4) = 4.6e-107
 Identities = 50/54 (92%), Positives = 50/54 (92%), Frame = +1

Query:     4 GKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKVRGLK 165
             GKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWK    K
Sbjct:     1 GKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSAK 54

 Score = 265 (93.3 bits), Expect = 4.6e-107, Sum P(4) = 4.6e-107
 Identities = 52/56 (92%), Positives = 52/56 (92%), Frame = +3

Query:   330 TMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPP*VSCLFL 497
             TMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPP  S  FL
Sbjct:    50 TMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPP--SAFFL 103

 Score = 80 (28.2 bits), Expect = 4.9e-31, Sum P(2) = 4.9e-31
 Identities = 18/60 (30%), Positives = 30/60 (50%), Frame = +2

Query:   971 SAFFLFCSEYRPKIKGEHPGLSIG--DVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEK 1144
             S++  F    R + K +HP  S+   + +KK  E W   +A +K  +E  A   K +YE+
Sbjct:    13 SSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSAKEKGKFEDMAKADKARYER 72

 Score = 65 (22.9 bits), Expect = 1.8e-29, Sum P(2) = 1.8e-29
 Identities = 14/45 (31%), Positives = 22/45 (48%), Frame = +1

Query:    13 DPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERW 147
             DP  P+   S++  F    R + K +HP  S+   + +KK  E W
Sbjct:    90 DPNAPKRPPSAFFLFCSEYRPKIKGEHPGLSIG--DVAKKLGEMW 132

 Score = 42 (14.8 bits), Expect = 1.3e-24, Sum P(2) = 1.3e-24
 Identities = 11/37 (29%), Positives = 19/37 (51%), Frame = +3

Query:   336 SAKEKGKFEDMAKADKARYEREMKTY-IPPKGETKKK 443
             +A +K  +E  A   K +YE+++  Y    K +  KK
Sbjct:   136 AADDKHPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKK 172

Parameters:

  ctxfactor=6.00
  E=10

  Query                        -----  As Used  -----    -----  Computed  ----
  Frame  MatID Matrix name     Lambda    K       H      Lambda    K       H
   Std.    0   BLOSUM62                                 0.318   0.135   0.401  
   +3      0   BLOSUM62        0.318   0.135   0.401    0.374   0.168   0.632  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +2      0   BLOSUM62        0.318   0.135   0.401    0.357   0.161   0.556  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +1      0   BLOSUM62        0.318   0.135   0.401    0.358   0.161   0.586  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -1      0   BLOSUM62        0.318   0.135   0.401    0.357   0.159   0.555  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -2      0   BLOSUM62        0.318   0.135   0.401    0.361   0.163   0.576  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -3      0   BLOSUM62        0.318   0.135   0.401    0.352   0.155   0.521  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a

  Query
  Frame  MatID  Length  Eff.Length     E    S W   T  X   E2     S2
   +3      0      857       857       10.  77 3  12 22  0.11    36
                                                    37  0.10    40
   +2      0      858       858       10.  77 3  12 22  0.11    36
                                                    37  0.10    40
   +1      0      858       858       10.  77 3  12 22  0.11    36
                                                    37  0.10    40
   -1      0      858       858       10.  77 3  12 22  0.11    36
                                                    37  0.10    40
   -2      0      858       858       10.  77 3  12 22  0.11    36
                                                    37  0.10    40
   -3      0      857       857       10.  77 3  12 22  0.11    36
                                                    37  0.10    40


Statistics:

  Database:  /dbEST1/wublast/database/swissprot/sprot.fa
    Title:  swissprot
    Release date:  unknown
    Posted date:  11:37 AM GMT Feb 21, 2000
    Format:  BLAST
  # of letters in database:  26,335,942
  # of sequences in database:  72,615
  # of database sequences satisfying E:  476
  No. of states in DFA:  599 (59 KB)
  Total size of DFA:  635 KB (640 KB)
  Time to generate neighborhood:  0.08u 0.00s 0.08t  Elapsed: 00:00:00
  No. of threads or processors used:  2
  Search cpu time:  216.45u 0.39s 216.84t  Elapsed: 00:01:53
  Total cpu time:  217.02u 0.56s 217.58t  Elapsed: 00:01:55
  Start:  Mon Feb 21 12:12:16 2000   End:  Mon Feb 21 12:14:11 2000

WARNINGS ISSUED:  1
